Getting started with imago
All information you need to start using imago in one place.
Download and install
Simple guide to download and install imago.
Download
- Go to the download page.
- Click Download button.
- Save to disk.
Install
- Double click on the imago installation file you downloaded.
- imago will check if .NET framework 3.5 and Visual Studio Runtime libraries have installed on your computer. Press Accept. (If .NET framework 3.5 is already installed, it won't install it again.)
- imago 1 Setup wizard will welcome you. Press Next.
- Accept the software license agreement. Choose I Agree and press Next.
- Install imago in the suggested directory. Press Next.
- The information is complete and the installation will begin. Press Next.
- imago can start automatically after installation. Press Close.
Organizing images in imago
When you first install Mayachitra imago, it displays an album viewer. You will create albums and organize the images within imago. You can browse all microscopic images in your hard drive and import them into albums. Mayachitra imago works independently from any imaging device. You can manage multiple image types (including Fluoview TIFF, OME TIFF, and MetaMorph STK) and the associated metadata in a single place. All different microscopic images can be stored in one, easy to use interface.
Importing images into imago
When you first start Mayachitra imago, it displays an empty list in the album viewer. You can easily create a new album and import images into the album. Albums exist only in imago. Your albums don't correspond to real folders on your hard drive. When you delete or move images from an album, the original files remain in their original locations on your hard drive.
Working with stack images
imago's image viewer provides you with an effective way to browse through the stack. There are different types of stacks: time lapse stack, z-stack (sequential optical cut through the same tissue), and combination of both time and z. imago's image viewer helps you to view all slices in a stack with its metadata (e.g. the number of z or t slices, the number of channels, and pixel resolution).
Easy and rich annotations
With imago, you can measure length, perimeter, area, and count cells from an image. imago's annotation tool will also help you to create a publication-ready image. The scale bar on an image is a single click away.
Quick and easy image processing
Remove noise, enhance the contrast of an image, resize an image, and check its histogram. It is simply easy with Mayachitra imago. Moreover, imago does not overwrite your images. imago always keep your original image intact. The changes you make can only be viewed in imago until you export or save the image with its changes.
Automatic image analysis
Now all your images are organized in Mayachitra imago. You can analyze them with imago's analysis tools.
Powerful automatic image registration
Mayachitra imago provides powerful registration functionality that automatically aligns multiple images and creates one mosaicked image. No parameter tuning is needed, nor any ordering of the images themselves. imago not only assembles multiple images automatically but also blends abutting edges while minimizing alteration of the original image. Your mosaic looks photo realistic and is perfectly suitable for further quantitative analysis.
Automatic nuclei detector
Mayachitra imago's automatic nuclei detector is a powerful image-based cell counter. It automatically identifies nuclei's centroid locations from the selected image and provides you the total number of cells and their locations. imago's nuclei detector is incredibly simple to use yet powerful and robust enough to analyze large sets of digital micrographs.
Interactive cell analysis
Mayachitra imago provides you with an interactive cell segmentation method that segregates the background from the foreground, given the information provided by the user in terms of mouse-drawn scribbles. The segmentation result is updated dynamically as a scribble is added or removed. imago computes the properties of individual cells such as area, perimeter, and average intensity.
Powerful unified search
Search the image you want to work with in one place by image name, annotation, your own tags, image content, or any combination of the above. You can spend more time analyzing and learning from images. Mayachitra imago helps you to organize your images based on search results. Search results can be easily grouped into a data set by user tags.
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