Powerful automatic image registration (mosaicking)
It is important to create a wide field-of-view image while maintain high resolution. A mosaic allows a wide field of view of a biological specimen without sacrificing resolution. Mayachitra imago provides you a powerful automatic image registration tool to create these mosaics.
No high-precision, motorized stage is required. As long as there is enough overlapping area between the images, imago's registration will create a mosaic automatically. No rough arrangement of images nor parameter tuning is required. Registration is done in two quick steps!
No high-precision, motorized stage is required. As long as there is enough overlapping area between the images, imago's registration will create a mosaic automatically. No rough arrangement of images nor parameter tuning is required. Registration is done in two quick steps!
If your image set is collected using a motorized stage, imago also takes advantage of extra information provided by the microscope (e.g. MATL log file). How to create a mosaic using a log file.
1. Select images to mosaic.
Select multiple images that you would like to mosaic from imago's album viewer.
Right click and choose the menu Register Selected Images.
What about stack images?
If the selected images are z-stacks, imago will offer you to create the projection images. Click Yes.
z-frame projected images
imago will show you the montage of z-frame projected images. If you like the projected images, Click Register images.
2. Enter a name for a new mosaic and choose a mapping.
Mayachitra imago will ask you the name of the resulting mosaicked image that will be added to the album and the settings to use.
- Type the name of the resulting mosaicked image.
- Select the registration setting.
- Click Start
Registration setting
- Mapping: Different ways to align images together. There are three different mappings.
Translation: Aligns the images and matches overlapping content, but does not transform (rotate, scale, stretch, or skew) any of the input images. This is the simplest mapping.
RST: Aligns the images and matches overlapping content and transforms (rotate, scale, or translate) any of the input images.
Homography: Aligns the images and matches overlapping content and transforms (rotate, scale, translate, stretch, or skew) any of the input images. This is the most complex mapping.
- Correct Lens Distortion: By turning on this option, fish-eye and other distortions caused by lenses are corrected. The image collected using confocal microscope usually does not induced lens distortion.
3. Done.
After a while the registration engine will finish its job and imago will add the mosaicked image to the album.
How to choose the right mapping for my dataset?
In most cases, the RST option works great on microscopic images so you'll definitely want to try that one first. If, for some reason, the RST option doesn't give you the results you expected, you can always try again with one of the other options.
Do you have images taken with different resolutions but still want to create a mosaic with them?
No problem! Mayachitra imago can handle up to 400% scale difference. For example, an image taken with 40x lens can be mosaicked with an image taken with 10x lens.
Copyright © 2006-2010 Mayachitra, Inc. All rights reserved.